rabbit anti cd34 primary antibody  (Servicebio Inc)

Journal: Journal of Nanobiotechnology

Article Title: Gut microbiota contributes to polystyrene nanoplastics-induced fetal growth restriction by disturbing placental nicotinamide metabolism

doi: 10.1186/s12951-025-03650-1

Figure Lengend Snippet: PS-NPs exposure induced placental injuries ( A ) Representative images of placentas from the control and different PS-NPs treatment groups (1, 10, and 100 mg/kg) at GD 18.5. ( B ) Histograms of placental weight ( n = 8). ( C ) Histograms of the placental/body weight ratio (%; n = 8). ( D ) Histograms of placental diameter ( n = 8). ( E ) Representative HE stained images of placental tissues (D: decidual, JZ: junctional zone, LZ: labyrinth zone, scale bar = 500 μm). ( F ) Percentages of the decidual, junctional and labyrinth zones relative to the total placental area. ( G ) Representative HE stained images of the blood sinus, scale bar = 50 μm. ( H ) Quantification of the blood sinus area. ( I ) Immunostaining images of CD34 in the mouse placenta, scale bar = 20 μm. ( J ) Quantification of CD34-positive area in mouse placenta. ( K ) Relative mRNA expression of Eomes , Ascl2 , Fra1 and Hand1 in various groups. ( L ) PCNA protein expression in different groups. ( M , N ) Relative mRNA and protein expression of ZO1 , Claudin1 and Occludin . * P < 0.05, ** P < 0.01

Article Snippet: After three washes with PBS, the sections were incubated with a recombinant rabbit anti-CD34 primary antibody (GB15001, Servicebio Technology Co., Ltd., Wuhan, China) at a dilution of 1:200 for immunohistochemical analysis.

Techniques: Control, Staining, Immunostaining, Expressing

Journal: Communications Biology

Article Title: Pseudogene MAPK6P4 -encoded functional peptide promotes glioblastoma vasculogenic mimicry development

doi: 10.1038/s42003-023-05438-1

Figure Lengend Snippet: a IP and IB analysis of KLF15 phosphorylation in HEK-293T cells transfected with the indicated constructs. b Half-life of KLF15 protein was measured in HEK-293T cells transfected with the indicated constructs after treatment with CHX. ** P < 0.01, compared with corresponding the WT group ( n = 3). c The nude mice carrying U251 and U373 cells suspension formed tumors in respective groups are shown. The sample tumors resected from respective groups are shown. d Tumor growth curves are shown. Tumor volume was calculated every 5 days after U251 and U373 cells suspension injection, and the tumor was resected at 30 days. Data are presented as the mean ± SD ( n = 8, each group). e Survival curves of nude mice U251 and U373 cells suspension injected into the right striatum are shown ( n = 8, each group, log-rank test). f Ki-67 immunohistochemical staining in orthotopic xenografts (×400; scale bar represents 20 μm). g CD34-PAS double staining was applied to detect the VM in orthotopic xenografts (×200; scale bar represents 50 μm). Data are presented as the mean ± SD ( n = 5, each group). ** P < 0.01, compared with WT group.

Article Snippet: IHC was performed using rabbit anti-human CD34 primary monoclonal antibody (Beijing Zhongshan Golden Bridge, China).

Techniques: Transfection, Construct, Suspension, Injection, Immunohistochemical staining, Staining, Double Staining

Journal: Communications Biology

Article Title: Pseudogene MAPK6P4 -encoded functional peptide promotes glioblastoma vasculogenic mimicry development

doi: 10.1038/s42003-023-05438-1

Figure Lengend Snippet: a The nude mice carrying U251 and U373 cells suspension formed tumors in respective groups are shown. The sample tumors resected from respective groups are shown. b Tumor growth curves are shown. Tumor volume was calculated every 5 days after subcutaneous injection of U251 and U373 cell suspensions in nude mice, and the tumor was resected at 45 days. Data are presented as the mean ± SD ( n = 8, each group). ** P < 0.01 compared with Control group, ## P < 0.01 compared with sh MAPK6P4 group; & P < 0.01 compared with shKLF15 group; % P < 0.01 compared with shLDHA group. c Kaplan-Meier survival curves of mice bearing orthotopic xenografts ( n = 8, each group, log-rank test). ** P < 0.01 compared with Control group, # P < 0.01 compared with sh MAPK6P4 group; & P < 0.01 compared with shKLF15 group; % P < 0.01 compared with shLDHA group. d Ki-67 immunohistochemical staining in orthotopic xenografts (×400; scale bar represents 20 μm). e CD34-PAS double staining was applied to detect the VM in orthotopic xenografts (×200; scale bar represents 50 μm). Data are presented as the mean ± SD ( n = 5, each group). ** P < 0.01 compared with Control group, ## P < 0.01 compared with sh MAPK6P4 group; & P < 0.05 compared with shKLF15 group; % P < 0.05 compared with shLDHA group.

Article Snippet: IHC was performed using rabbit anti-human CD34 primary monoclonal antibody (Beijing Zhongshan Golden Bridge, China).

Techniques: Suspension, Injection, Control, Immunohistochemical staining, Staining, Double Staining